教授

  • 姓名:刘德立
  • 职称:教授
  • 研究领域:1. 基因工程和应用酶学研究。2. 环境微生物研究。
  • 联系方式:ldl(AT)mail.ccnu.edu.cn; deliliu2013(AT)163.com
  • 办公室地址:生科院338室
  • 更多信息:

教育经历:

1975.9—1978.7 华中师范大学生物学 学士

1978.9—1981.7 华中师范大学化学系 进修

1996.9—1999.6 武汉大学生命科学院 博士

工作经历:

1978.8—1986.6 华中师范大学生科院 助教

1986.6—1993.6 华中师范大学生科院 讲师

1993.6—1998.12华中师范大学生科院 副教授

1998.12—. 华中师范大学生科院 教授

国外工作经历:

1986.9—1987.12美国俄亥俄州立大学生物化学系访问学者

1993.9—1995.4日本筑波大学应用生物化学系 访问学者

2000.8—2002.3日本筑波大学应用生物化学系 研究员

2002.4—2004.1日本神户大学遗传研究中心 研究员

荣誉或奖励:

2009年10月获湖北省教学成果一等奖(排名第二)湖北省教育厅

新世纪跨学科“化学生物学”人才培养模式与课程体系改革的研究与实践

2011年12月获湖北省自然科学三等奖(排名第一)湖北省政府

环境微生物功能酶降解机理与新型农残检测技术研究

主要研究领域:

1.基因工程和应用酶学研究。主要从事细胞色素P450的分子生物学研究,包括真菌反硝化相关的P450nor作用机理和农药靶标酶CYP51的作用机理研究。

2.环境微生物研究。在环境治理方面,着重开展了微生物脱氮(反硝化)、有机磷农药降解机理的研究工作。

承担科研项目:

1. 国家自然科学基金面上项目(31371893):柑橘霉菌农药靶标酶CYP51转录调控及其抗药性分子机理研究2014.1-2017.12 (主持)

2. 国家自然科学基金面上项目(31071653):柑橘青霉菌农药靶标酶CYP51活性部位结构与抑制机理研究2011.1-2013.12 (主持)

3. 国家自然科学基金面上项目(30771429):真菌细胞色素P450-14DM基因表达调控与农药抗性机理研究 2008.1-2010.12 (主持)

4. 国家科技部“863”项目(2007AA05Z417):生物柴油新型脂肪酶及催化工艺研究2008.7-2010.12 (副组长)

5. 国家自然科学基金面上项目(30170011):真菌细胞色素P-450nor与反硝化作用机理研究2002.1-2004.12 (主持)

6. 国家科技部973前期项目(2004CCA00100):基于靶酶结构及酶催化机理的光学活性农药的合理设计及其对称选择性合成——负责“真菌细胞色素P45014-DM酶基因的克隆表达的研究” 2005.7–2006.10 (排名第二)

7. 教育部博士点基金项目(20060511002):反硝化真菌脱氮机理及其一氧化氮还原酶的功能研究 2006.1-2008.12 (主持)

代表性论文:

1. NiuYH, Zhang TF, Zhu Yi, YuanYZ, Wang SQ, Liu J,Liu DL*.Isolationandcharacterizationofanovelmycovirusfrom Penicillium digitatum.Virology2016,494:15–22

2. ZhangJH, Zhao L, Zhang J, Han R, Li SX, Yuan YZ, Wan J, Xiao WJ andLiu DL*.Optimisedexpression and spectral analysis of the target enzyme CYP51 fromPenicilliumdigitatumwith possible new DMI fungicides. PestManag Sci. 2010, 66: 1344–1350

3. HanR, Zhang JH, Li SX, Cao SF, Geng H, Yuan YZ, Xiao WJ, Liu SH andLiu DL*.Homology Modeling and Screening of New 14a-DemethylaseInhibitor (DMI) Fungicides Based on Optimized Expression of CYP51 from Ustilagomaydis inEscherichia coli.J Agric Food Chem.2010,58, 12810–12816 

4. LiWJ, Li Q,Liu DL*,and Ding MW* Synthesis, Fungicidal Activity,and Sterol 14α -Demethylase Binding Interaction of 2‑Azolyl-3,4-dihydroquinazolines onPenicilliumdigitatum.J Agric Food Chem.2013,61:1419-1426

5. WeiyingZhang, Yong Tang, Dan Du*, Jordan Smith, Charles Timchalk,Deli Liu*,Yuehe Lin*. Direct analysis of trichloropyridinol in human saliva using an Aunanoparticles-based immunochromatographic test strip for biomonitoring ofexposure to chlorpyrifos. Talanta2013,114:261-267

6. WeiyingZhang1,3, Xiaoxiao Ge2, Yong Tang2, Dan Du2,3,Deli Liu1*, Yuehe Lin3*.Nanoparticle-BasedImmunochromatographic Test Strip with Fluorescent Detector for Quantificationof Phosphorylated Acetycholinesterase: An Exposure Biomarker ofOrganophosphorous Agents. Analyst2013,138, 5431-5436

7. JingLiu, Yongze Yuan,Zhi Wu, Na Li, YuanleiChen, Tingting Qin, Hui Geng, Li Xiong,Deli Liu*A Novel SterolRegulatory Element-Binding Protein Gene ( sreA ) Identified in PenicilliumdigitatumIs Required for Prochloraz Resistance, Full Virulence anderg11(cyp51)Regulation.PLoS ONE2015, 10(2): e0117115.

8. JingLiu‡, Shengqiang Wang‡, Tingting Qin, Na Li,Yuhui Niu, DandanLi, Yongze Yuan, Hui Geng, Li Xiong, DeliLiu* Wholetranscriptome analysis ofPenicillium digitatumstrains treatmented withprochloraz reveals their drug-resistant mechanisms.BMCGenomics2015, 16:855 DOI:10.1186/s12864-015-2043-x

9. Zhi Wu.ShengqiangWang.Yongze Yuan.TingfuZhang.Jing Liu.DeliLiu*.A novel major facilitator superfamily transporter in Penicillium digitatum(PdMFS2) is required for prochloraz resistance, conidiation and full virulence.Biotechnol Lett2016,38:1349–1357 DOI10.1007/s10529-016-2113-4.

10. Dandan Li1,Jiali Yan1, Li Wang, Yunze Zhang,DeliLiu, Hui Geng, Li Xiong*. Characterization of the phthalate acidcatabolic gene cluster in phthalate acid esters transforming bacterium-Gordoniasp. Strain HS-NH1,International Biodeterioration & Biodegradation,2016,106:36-40. DOI: 10.1016/j.ibiod.2015.09.019.

11. Na Li12*, Yunze Zhang1*,Dandan Li1, Yuhui Niu1, Jing Liu1, Shuxiang Li1,Yongze Yuan1, Shuli Chen1, Hui Geng1,DeliLiu1**.Overexpression, homology modeling and coenzyme dockingstudies of the cytochrome P450nor2 fromCylindrocarpon tonkinense,MolecularBiology,2016,50(2):320–327.

12. LiN,*Li D.D*, Zhang Y.Z, Yuan Y.Z, Geng H, Xiong L and Liu D.L. Genome sequencingand systems biology analysis of a lipase-producing bacterial strain,Genet.Mol. Res.2016, 15(1): gmr.15017331

13. PanWang Yongze Yuan Qian Li Junzhong Yang Yongliang Zheng Muqing He Hui Geng LiXiongDeli Liu* Isolation and immobilization of new aerobic denitrifyingbacteria.InternationalBiodeterioration & Biodegradation2013,76:12-17

14. XueYang, Can Zhang, Zhen He, Xiaojing Hu, Jia Guo, Qiu Zhong, Jinlong Wang, LiXiong*,Deli Liu. Isolation and characterization of two n-butyl benzylphthalate degrading bacteria,International Biodeterioration &Biodegradation.2013,76:8-11

15. Cao XF,Hu M, Zhang J, Li F, Yang YH,Liu DL*,Liu SH*.Determination of StereoselectiveInteraction between Enantiomers of Chiral γ-Aryl-1H-1,2,4-triazole Derivativesand Penicillium digitatum.J. Agric. Food Chem.2009, 57,6914–6919

16. Du D*,Ye XP, Zhang JD, Zeng Y, Tu HY, Zhang AD,Liu DL*.Stripping voltammetricanalysis of organophosphate pesticides based on solid-phase extraction atzirconia nanoparticles modified electrode.Electrochem. Commun.2008, 10, 686-690.

17. Dan Du*,Xiuping Ye, Jiande Zhang,Deli liu*(刘德立).Cathodicelectrochemical analysis of methyl parathion at bismuth-film-modified glassycarbon electrode.Electrochimica Acta2008, 53,4478-4484.

18. DanDu, Wenjuan Chen,Weiying Zhang,Deli Liu Haibing Li, YueheLin. Covalent coupling of organophosphorus hydrolase loaded quantum dots tocarbon nanotube/Au nanocomposite for enhanced detection of methyl parathion.Biosens. Bioelectron2010,25:1370-1375

19. Weiying Zhang,Jiawang Ding, Yuehua Qin,Deli Liu*and Dan Du* One-Step Electrochemically Deposited GoldNanoparticles Interface Grafted with Avidin for Acetylcholinesterase BiosensorDesign. Journal of Nanoscience and Nanotechnology2010,10, 1–7.

20. Qingye Zhang§†‡, Ding Li§†,Pei Wei†, Jie Zhang†, Jian Wan*†, Yangliang Ren†, Zhigang Chen†,Deli Liu†,Ziniu Yu†‡ and Lingling Feng*† Structure-Based Rational Screening of Novel HitCompounds with Structural Diversity for Cytochrome P450 Sterol 14α-Demethylase from Penicilliumdigitatum J. Chem. Inf. Model.,2010, 50 (2),pp 317–325

21. Shuxiang Li, Jianhua Zhang,Shufen Cao, Rui Han, Yongze Yuan, Jiangke Yang, Yunjun Yan,Deli Liu*.Homology modeling,molecular docking and spectra assay studies of sterol 14a-demethylasefromPenicillium digitatum. Biotechnol Lett2011 33:2005-2011

22. Dan Du∗, YuanTao, Weiying Zhang,Deli Liu, Haibing Li.Oxidativedesorption of thiocholine assembled on core–shell Fe3O4/AuNPsmagnetic nanocomposites for highly sensitive determination ofacetylcholinesterase activity: An exposure biomarker of organophosphates.Biosensorsand Bioelectronics201126:4231–4235

23. LiZhao,Deli Liu*, Qingye Zhang, ShanZhang, Jian Wan & Wenjing Xiao. Expression and homology modeling of sterol14a-demethylase fromPenicilliumdigitatum.FEMSMicrobiol Lett.2007, 277:37–43

24. Zheng YL,Liu DL*,Xu H, Zhong YL, Yuan YZ, Xiong L, Li WX.Biodegradation of p-nitrophenol by Pseudomonasaeruginosa HS-D38 and analysis of metabolites with HPLC-ESI/MS.IntBiodeterior & Biodegrad 2009, 63: 1125-1129

25. Ya-LiBEN1,3,Gu-ZhenCUI1, ChenLI1,RuiHAN1, JieZHANG1, Qie-YeZHANG2, JianWAN2, De-LiLIU*. Expression, Purification,Characteristics and Homology Modeling of the HMGS fromStreptococcuspneumoniae. Biomedical and Environmental Sceince2009,22: 229-236

26. ZHENGYongliang,LIU Deli*, LIU Shiwang, XU Shangying, YUAN Yongze, XIONGLi. Kinetics and mechanisms of p-nitrophenol biodegradation byPseudomonas aeruginosa HS-D38 Journal of Environmental Sciences 2009 21(9):1194-1199

27. QingY. Zhang, Kai Liu, Guzhen Cui, Xiang Y. Lin, Jian Wan, andDe L Liu.Interactionsof Ligands with Class-II HMG-CoA Reductase ofStreptococcus Pneumoniaeby Homology Modeling and Molecular Dynamic Simulations.Journal ofComputational and Theoretical Nanoscience, 2007, 4:1-6

28. LIUDL*, ZHANG S, WANG W,SHOUN H. Denitrificationby the Mix-culturing of Fungi and Bacteria with Shell.MicrobiologicalResearch.2006,161:132-137

29. LIP,LIU DL*,NAHIMANA L, CHEN SL, YANG X and ZHAO L. High nitrogenremoval from wastewater with several new aerobic bacteria isolated from diverseecosystems.J Environ Sci.2006 18(3):525-529

30. BenYali,Liu Deli*, Dali Zhu, Derui Zhu and Qin Luo. The Difference of Sensitivity between BXPC-3 and K562 Cells by Treatments withCombination of Indole-3-acetic Acid and Horseradish PeroxidaseJ. WuhanUniv. Technology (Materials Science Eidt)2006, 21(4):95-98

31. 李书祥韩睿袁利玲熊丽袁永泽杨江科闫云君刘德立*玉米黑粉菌CYP51稀有密码子和mRNA二级结构分析及同源模建与分子对接生物化学与生物物理研究进展 201138:751-757

32. 朱德锐韩睿沈国平龙启福李丹丹刘建刘德立.青海湖盐单胞菌Ectoine合成基因簇ectABC的重组共表达 水生生物学报 2015, 39(2):351-360

33. 韩睿陈来生 李莉 刘德立 PCR-DGGE研究青海农村户用沼气池微生物群落结构 中国环境科学2015,35(6): 1794-1804

34. 吴平高谦刘德立付友袁永泽文春根. 鲤TYK2基因的克隆鉴定及组织表达分析水生生物学报2015, 39(1):237-241

35. 牛玉慧刘婧齐婷伍志秦婷婷李青钟靖然张恒王崇武袁永泽刘德立*柑橘青霉菌CYP51B基因和上游调控区克隆及生物信息学分析微生物学通报2016,43(1): 76−87

36. 秦婷婷,耿辉,刘德立*等。指状青霉cytb5cyt b5r基因克隆及与cyp51A的共表达。生物技术通报2016, 32(9):1-6

37. 张建华熊丽夏诗慧王金龙李倩刘德立*柑橘绿霉菌CYP51的同源模建和定点突变分析植物保护学报2012 39(1):81-86.

38. 倪砚高谦刘德立*韦友传付建平胡炜汪亚平聂品 鲤鱼SOCS-4基因克隆、鉴定及表达模式分析水生生物学报2012 36(3):541-545

39. 韩睿邓灵福黎晨张青叶张劼高强熊丽万坚刘德立*玉米黑粉菌CYP51基因结构分析及突变表达条件优化 生物工程学报 2008 24(10):1-7

40. 张劼 黎晨赵莉韩睿万坚刘德立* 新型含氮化合物对柑桔绿霉菌的离体抑菌活性及构效关系研究 植物保护学报 2008 35(6):513-518

41. 王彬彬熊丽郑永良张志霞童文羽刘松陈奕和肖文精刘德立* 甲基对硫磷高效降解菌的分离鉴定及降解酶基因的克隆表达 环境科学学报 2008 28(10):1-7

42. 杨希,刘德立*,邓灵福,朱德锐,高强,魏艳红,刘松,邹煜平,熊丽蜡状芽孢杆菌好氧反硝化特性研究 环境科学研究 2008 21(3):155-159

43. 贲亚琍刘德立*朱德锐胡芹芹呋喃丹多克隆抗体的制备与初步应用 环境科学研究2008 21(3):175-179

44. 魏艳红熊鹰袁永泽 郑扬忠邓子厚 张华李风 杨江科闫云君刘德立*纤维素酶产生菌HS-F9的筛选鉴定和产酶条件优化应用与环境生物学报2010 162):274-278

45. 杨俊忠倪砚许尚营徐可瀚刘义曾丽霞刘德立*一株高效好氧反硝化菌的分离及特性研究生物学通报2010 37(11):1-6

46. 许尚营张华李娜李风杨俊忠郑永良刘德立* 一株好氧反硝化菌的鉴定及反硝化特性研究环境科学与技术 2010 33(2):10-13

出版著作:

1.张楚富,程汉华,刘德立(副主编)《生物化学原理》高等教育出版社 2011

2.禹帮超 胡耀星刘德立《酶工程》(第二版)华中师范大学出版社2007

3.熊丽丁书茂罗勤袁均林刘德立《生物化学与分子生物学实验》

华中师范大学出版社2007

4.禹帮超刘德立《应用酶学导论》华中师范大学出版社1995

5.刘德立参编《基因生物学——基因“一生”的轨迹》中国医药科技出版社2011

专利:

1.丁明武;刘德立;李文菁.2-(1H-咪唑-1-基)喹唑啉衍生物的制备与杀菌活性[P].CN201110306387.X.华中师范大学.2012-01-18 2013.6.5授权 专利号:ZL2011 1 0306387.X

2.丁明武;刘德立;和平.3-(1H-咪唑-1-基)喹啉衍生物的制备与杀菌活性[P].CN201110306404.X.华中师范大学.2012-04-11 2013.4.17授权 专利号:ZL2011 1 0306404.X

学术兼职:

兼任湖北省生物化学与分子生物学学会常务理事和湖北省生物工程学会常务理事。